Summary
The Forsyth Institute Multiplex Core has expertise in a wide range of sample types. We only need from 2.5 to 50 µl* per sample to detect numerous markers of interest. Assays are available on 2 platforms: Luminex200 and BioPlex3D. We have the capability to customize kits based on your project needs. (* sample volume is assay-dependent)
We offer “Full Service” or “Read Only” analysis options for researchers and companies interested in working with our Core.
Mission
To provide researchers access to multiplex and/or ELISA analysis of any sample type using commercially available or inhouse developed assays. To provide researchers access to our extensive knowledge and experience with various sample types and previously assayed biomarkers.
Services Offered
Full-service Luminex or ELISA sample analysis:
- Core works with researcher to plan out project needs, panel configurations, sample volumes, etc.
- Core provides full service quote
- Core purchases kits and materials needed
- Core performs kit preparation and data acquisition
- Core provides organized data as excel file to researcher
Read-Only Luminex or ELISA analysis
A cost-effective service option for researchers confident in performing kit preparation and simply need a platform to acquire data.
- Core works with researcher to plan out project needs, panel configurations, sample volumes, etc.
- Core provides a Read Only quote
- Researcher purchases kits and materials needed
- Researcher performs kit preparation and brings finished plate to the Core
- Core personnel performs the data acquisition
- Core provides organized data as excel file to researcher
Equipment
Luminex 200 platform
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The standard Luminex workhorse.
- Plex up to 100 markers in one well
- Annual service contract, regular and preventive maintenance
BioPlex 3D platform
- Plex up to 500 markers in one well (requires customized assays)
- 384-well plate capabilities
- Annual service contract, regular and preventive maintenance
Bio-Rad Magnetic Plate Washer
KingFisher Flex Magnetic automated instrument
Allows for “sequential luminex” of extremely limited volume samples, ask for more details!
Additional Information
Multiplex immunoassay background
- A platform for simultaneous, multiplex detection of multiple proteins in a single biological sample using a small sample amount (<50uL)
- Basic concept:
- Formation of a “capture antibody-analyte-fluorescent labeled detection antibody” sandwich attached to fluorescently labeled microsphere beads read by flow-based laser technology
- Sample Types
- Various species including but not limited to human, mouse, rat, non-human primate, porcine, etc
- The Core has extensive experience with many sample types including serum/plasma, saliva, Gingival Crevicular Fluid (GCF), air pouch model, cell culture supernatant, cell lysates, tissue lysates, wound fluid, urine, and others.
- Immunoassay portfolio
- The Core has run a majority of the commercially available panels
- Specific areas of interest include immunology, cancer, cardiovascular disease, cell signaling, metabolism/endocrinology, neuroscience, toxicity, and others.
- Our most trusted panel manufacturers work closely with us to customize panels, optimize conditions, and provide our customers with the best quality kits to produce the best quality results.
- Links to MilliporeSigma and R&D Systems
How to choose the right kits
The Core is happy to help you find and design the optimal panel configuration to meet all your research needs, reach out to request a quote or help.
Acknowledging the Core
If you publish data obtained through the core, please cite in the methods or acknowledgements: “Multiplex data were acquired at the Forsyth Institute Multiplex Core Facility (RRID:SCR_021783).”
