The ADA Forsyth Institute’s Core Facilities and Services provides specialized equipment, training and expertise on a fee-for-service basis to researchers from external institutions. AFI’s state-of-the-art facilities, advanced technologies, and experienced core managers can accommodate your project needs.
The ADA Forsyth Institute’s Flow Cytometry core facilities manage advanced flow cytometers and cell sorters, offering consultation and support services for the detection of up to 14 different fluorescent parameters simultaneously, RBC contaminated samples, and high-throughput analysis using 96 or 384-well plates.
The cell sorter accommodates collection of cells or particulates, after-screening for positively selected markers, downstream applications, and can sort four separate cell populations simultaneously.
Access to Flow Cytometry Core equipment is available at hourly rates or by sample, with training and demonstrated proficiency required. Please request more information by contacting flowcytometry@forsyth.org.
Equipment
Beckman Coulter CytoFLEX SRT Benchtop Cell Sorter
- Meets requirements for a wide range of cell sorting needs
- Capable of complex sort logic with different combinations of sort settings on each of four streams, including the ability to catch aborts of one of the other streams
- Powerful optics that allow for detection and sorting of nanoparticles down to 100 microns
- Optical innovations through wavelength division multiplexing and improved light collection
- Mixed mode sorting
- Sorts four populations simultaneously in tubes or deposits onto plates or slides
- Highly automated operation and error resolution
Invitrogen™ Attune™ NxT Flow Cytometer
- Features acoustic-assisted hydrodynamic focusing, which allows for sample-throughput rates up to 10 times faster than traditional cytometers.
- Using violet side scatter detectors it is possible to run no-wash, no-lyse protocols with RBC contaminated samples.
- 96 and 384 well plate autosampler for high throughput analysis.
- Our current filter setup allows for analyzing up to 14 separate colors/cell markers from a single sample
Additional Services Information
What is Flow Cytometry?
Flow Cytometry (abbreviated: FCM) is a technique for counting and examining microscopic particles, such as chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second. Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in both research and clinical practice. A common variation is to physically sort particles based on their properties, so as to purify populations of interest. This technique is known as fluorescence activated cell sorting (FACS), which uses fluorescent labels as a method for detection.
Pricing
Forsyth External Flow Core Pricing
| Attune NxT Flow Cytometer | ||
| Fees | Additional info | |
| Tube | $8/tube | Minimum $40 charge for less than 5 samples |
| 96 Well plate | $8/well | Maximum charge: $240 per plate |
| 384 Well Plate | $8/well | Maximum charge: $720 per plate |
| Include all conditions assayed in total reported count | ||
| CytoFLEX SRT Cell Sorter | |
| Hourly Rate | Additional Fees |
|
$200/hour |
· +$100 Start up/QC/Shutdown per session.
· + $200 Aseptic Sort Prep Fee. |
| Other Flow Core Resources | ||
| Attune or CytoFLEX SRT Training |
USB Drive Purchase | Flow Jo Dongle Rental |
| $100/hour | $6 per drive | $30/daily
(Max 3 days per sign off) |
Research Applications for Flow Cytometry
- Apoptosis
- Phagocytosis
- Bacteria cell count/viability
- T-cell cytokine expression
- Bead based immunoassays
- Antibody screening
- RNA gene expression
Protein phosphorylation
Flow Cytometry Core Services
Kyle Bredin has managed ADA Forsyth’s Flow Cytometry Core Facilities and Services for over four years. He can assist with contracting, training, and more.
